I.P. Pavlov Journal of Higher Nervous ActivityI.P. Pavlov Journal of Higher Nervous ActivityЖурнал высшей нервной деятельности им. И.П. Павлова0044-46773034-5316The Russian Academy of Sciences65201910.31857/S0044467723050118EUAPVXФИЗИОЛОГИЧЕСКИЕ МЕХАНИЗМЫ ПОВЕДЕНИЯ ЖИВОТНЫХ: ВОСПРИЯТИЕ ВНЕШНИХ СТИМУЛОВ, ДВИГАТЕЛЬНАЯ АКТИВНОСТЬ, ОБУЧЕНИЕ И ПАМЯТЬФИЗИОЛОГИЧЕСКИЕ МЕХАНИЗМЫ ПОВЕДЕНИЯ ЖИВОТНЫХ: ВОСПРИЯТИЕ ВНЕШНИХ СТИМУЛОВ, ДВИГАТЕЛЬНАЯ АКТИВНОСТЬ, ОБУЧЕНИЕ И ПАМЯТЬTHE CONTRIBUTION OF EPIGENETIC MECHANISMS TO THE FORMATION, MAINTENANCE AND RECONSOLIDATION OF THE LONG-TERM FOOD AVERSIVE MEMORY IN TERRESTRIAL SNAILВклад эпигенетических механизмов в формирование, поддержание и реконсолидацию долговременной пищевой аверсивной памяти виноградной улиткиZuzinaА. B.ЗюзинаА. Б.lucky-a89@mail.ruBalabanP. М.БалабанП. М.lucky-a89@mail.ruInstitute of Higher Nervous Activity and Neurophysiology, Russian Academy of ScienceФедеральное государственное бюджетное учреждение науки Институт высшей нервной деятельности и нейрофизиологии РАН0109202373568870302022025Copyright ©; 2023, А.Б. Зюзина, П.М. БалабанCopyright ©; 2023, А.Б. Зюзина, П.М. Балабан2023А.Б. Зюзина, П.М. БалабанА.Б. Зюзина, П.М. Балабанhttps://innoscience.ru/0044-4677/article/view/652019

This work was devoted to the analysis of the role of epigenetic mechanisms (histone acetylation, DNA methylation, histone serotonylation) in the formation, storage, and reconsolidation of long-term food aversive memory in the snail. In the first part of the work, we investigated the effect of systemic administration of sodium butyrate, an inhibitor of histone deacetylase, in a model of conditioned food aversion reflex of the snail. We have shown that the administration of sodium butyrate to animals with poor memory resulted in memory enhancement. Further, in experiments using the DNA methyltransferase blocker RG108, it was found that impaired DNA methylation impairs long-term food aversive memory in the snail. However, memory reactivation neutralizes the effect of inhibiting the activity of DNA methyltransferases. In the final section of the work, the hypothesis of the involvement of transglutaminase-mediated serotonylation in the processes of reconsolidation of food aversive memory in the snail was tested. Behavioral analysis showed that administration of the transglutaminase blocker monodansylcadaverine (hence, blockade of serotonylation) after a reminder disrupted the reconsolidation process and led to memory suppression/erasure.

Настоящая работа направлена на анализ роли эпигенетических механизмов (ацетилирование гистонов, метилирование ДНК, серотонилирование гистонов) в формировании, хранении и реконсолидации долговременной пищевой аверсивной памяти виноградной улитки. В первой части работы мы исследовали влияние системного введения ингибитора гистондеацетилаз бутирата натрия в модели условно-рефлекторной пищевой аверсии виноградной улитки. Мы показали, что введение бутирата натрия животным со слабой памятью приводило к усилению памяти. Далее в экспериментах с применением блокатора ДНК метилтрансфераз RG108 было обнаружено, что нарушение метилирования ДНК ухудшает долговременную пищевую аверсивную память у виноградной улитки. Однако, реактивация памяти нивелирует эффект ингибирования активности ДНК метилтрансфераз. В заключительном разделе работы была проведена проверка гипотезы о вовлечении трансглутаминаза-опосредованного серотонилирования в процессы реконсолидации пищевой аверзивной памяти виноградной улитки. Поведенческий анализ показал, что введение блокатора трансглутаминазы монодансилкадаверина (следовательно, блокада серотонилирования) после напоминания нарушало процесс реконсолидации и приводило к подавлению/стиранию памяти.

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