I.P. Pavlov Journal of Higher Nervous ActivityI.P. Pavlov Journal of Higher Nervous ActivityЖурнал высшей нервной деятельности им. И.П. Павлова0044-46773034-5316The Russian Academy of Sciences65206710.31857/S0044467724060084МЕТОДИКАResearch ArticleChronic imaging of dendritic spine morphology in 5xFAD-M hybrid line mice of Alzheimer’s disease modelХроническая визуализация морфологии дендритных шипиков у трансгенных мышей гибридной линии 5XFAD-M, являющихся моделью болезни АльцгеймераMatukhnoA. E.МатухноА. Е.
Russian Federation

Research Center for Neurotechnology

Научно-исследовательский технологический центр нейротехнологий

aematuhno@sfedu.ruTkachevaP. V.ТкачеваП. В.
Russian Federation

Research Center for Neurotechnology

Научно-исследовательский технологический центр нейротехнологий

aematuhno@sfedu.ruVoinovV. B.ВойновВ. Б.
Russian Federation

Research Center for Neurotechnology

Научно-исследовательский технологический центр нейротехнологий

aematuhno@sfedu.ruLysenkoL. V.ЛысенкоЛ. В.
Russian Federation

Department of Physics

Физический факультет

aematuhno@sfedu.ruEvsyukovaE. V.ЕвсюковаЕ. В.
Russian Federation

Research Center for Neurotechnology

Научно-исследовательский технологический центр нейротехнологий

aematuhno@sfedu.ruTaisaevaA. O.ТайсаеваА. О.
Russian Federation

Research Center for Neurotechnology

Научно-исследовательский технологический центр нейротехнологий

aematuhno@sfedu.ruBezprozvannyI. B.БезпрозванныйИ. Б.
Russian Federation

Research Center for Neurotechnology; Laboratory of Molecular Neurodegeneration

Научно-исследовательский технологический центр нейротехнологий; Лаборатория молекулярной нейродегенерации

aematuhno@sfedu.ruSouthern Federal UniversityЮжный федеральный университетPeter the Great Saint Petersburg State Polytechnic UniversityСанкт-Петербургский политехнический университет Петра Великого1112202474674275502022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://innoscience.ru/0044-4677/article/view/652067

Alzheimer’s disease (AD) is a chronic neurodegenerative disorder of neural structures in different areas of the brain. Loss of synapses is a key stage in the development of AD and it precedes significant loss of neurons. However, the mechanisms of synapse loss are uncertain. Structural and functional changes in synapses are interrelated with the morphology of postsynaptic formations – dendritic spines. This paper describes the implementation of the technology of chronic imaging of dendritic spines in transgenic animals using the methods of multiphoton fluorescence microscopy. Mice of the 5xFAD-M hybrid line were used. 5xFAD-M was derived by crossing transgenic mice with expressions of green fluorescent protein GFP in individual neurons of the brain (M-Line) and a mouse model of AD (5xFAD line). Methodological achievements revealed the multi-day dynamics of the density of dendritic spines in M-Line and 5xFAD-M mice. Transformations of morphological types of spikes were revealed during a long period of observations.

Болезнь Альцгеймера (БА) является хроническим нейродегенеративным заболеванием с поражением нейронных структур в разных областях головного мозга, включая утрату синапсов. Причины деструктивных изменений и гибели нейронов изучены недостаточно. Механизмы утраты синапсов неопределенны. При развитии БА происходят структурные и функциональные изменения в синапсах, которые взаимосвязаны с морфологией постсинаптических образований – дендритных шипиков. В работе описана технология хронической визуализации дендритных шипиков у трансгенных животных с помощью методов мультифотонной флуоресцентной микроскопии. Были использованы гибридные животные (5xFAD-М) с экспрессий зеленого флуоресцентного белка и моделью БА. Приведенные в работе методические решения позволили выявить многодневную динамику плотности дендритных шипиков, а также отследить преобразование их морфологических типов в течение длительного периода наблюдений.

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