I.P. Pavlov Journal of Higher Nervous ActivityI.P. Pavlov Journal of Higher Nervous ActivityЖурнал высшей нервной деятельности им. И.П. Павлова0044-46773034-5316The Russian Academy of Sciences68758510.31857/S0044467725040098ФИЗИОЛОГИЧЕСКИЕ МЕХАНИЗМЫ ПОВЕДЕНИЯ ЖИВОТНЫХ: ВОСПРИЯТИЕ ВНЕШНИХ СТИМУЛОВ, ДВИГАТЕЛЬНАЯ АКТИВНОСТЬ, ОБУЧЕНИЕ И ПАМЯТЬФИЗИОЛОГИЧЕСКИЕ МЕХАНИЗМЫ ПОВЕДЕНИЯ ЖИВОТНЫХ: ВОСПРИЯТИЕ ВНЕШНИХ СТИМУЛОВ, ДВИГАТЕЛЬНАЯ АКТИВНОСТЬ, ОБУЧЕНИЕ И ПАМЯТЬResearch ArticleThe influence of long term housing in enriched environment on the social behavior of rats and the expression of genes associated with neuroinflammationВлияние содержания в обогащенной среде на социальное поведение крыс и экспрессию генов, связанных с нейровоспалениемPavlovaI. V.ПавловаИ. В.
Russian Federation
pavlovfml@mail.ruBroshevitskayaN. D.БрошевицкаяН. Д.
Russian Federation
pavlovfml@mail.ruShvadchenkoA. М.ШвадченкоА. М.
Russian Federation
pavlovfml@mail.ruInstitute of Higher Nervous Activity and Neurophysiology RASФедеральное государственное бюджетное учреждение науки Институт высшей нервной деятельности и нейрофизиологии РАН150820257544985121507202515072025Copyright ©; 2025, Russian Academy of SciencesCopyright ©; 2025, Российская академия наук2025Russian Academy of SciencesРоссийская академия наукhttps://innoscience.ru/0044-4677/article/view/687585

The aim of the work was to find out whether prolonged housing in an enriched environment is able to reduce the negative effects of early proinflammatory stress on the social behavior of adult male rats. On the 3rd and 5th postnatal days (PND), the rats were injected with either lipopolysaccharide at a dose of 50 mcg/kg (LPS group) or saline solution (SAL group). Starting from 30 PND and until the end of the experiments, one part of the rats lived in an enriched environment (EE), and the other in standard conditions (STD). Testing of pairs of rats in the social dominance tube test revealed a lower number of victories in the SAL and LPS groups when contained in the OS than in similar groups of rats under STD conditions. The dominant properties of males of the LPS group in SAL-LPS pairs weakened under the influence of EE. In the resident intruder test, the number of aggressive attacks, offensive uprights, sexual attacks, and the time of intruder persecution decreased in males of the LPS group when kept in the EE, while the number of attacks increased in rats of the SAL group, on the contrary. Analysis of cytokine gene expression in SAL rats, but not in the LPS group, showed an increase in the expression of anti-inflammatory cytokine TGF-beta1 mRNA in the dorsal hippocampus, amygdala and proinflammatory cytokine IL-1beta in the prefrontal cortex after housing in the EE. Thus, the housing in the EE had a beneficial effect on the behavior of the rats of the LPS group, reducing the dominant properties and aggressive behavior.

Целью работы было выяснить, способно ли длительное содержание в условиях обогащенной среды уменьшить негативные последствия раннего провоспалительного стресса в социальном поведении взрослых крыс-самцов. На 3-й и 5-й постнатальные дни (ПНД) крысам Вистар вводили либо липополисахарид в дозе 50 мкг/кг (группа ЛПС), либо физиологический раствор (группа ФИЗ). Начиная с 30-го ПНД и до конца экспериментов одна часть крыс проживала в условиях обогащенной среды (ОС), а другая – в стандартных условиях (СТАНД). Тестирование пар крыс в трубе на социальное доминирование обнаружило при содержании в ОС меньшее число побед у групп ФИЗ и ЛПС, чем у аналогичных групп крыс в СТАНД-условиях. Самцы группы ЛПС в парах ФИЗ-ЛПС переставали выигрывать под воздействием ОС. В тесте «резидент-интрудер» у самцов группы ЛПС при содержании в ОС было ниже число нападений, стоек, число сексуальных атак, время преследования интрудера, а у крыс группы ФИЗ число нападений, наоборот, было больше, чем при содержании в СТАНД-условиях. Анализ экспрессии генов цитокинов показал у крыс ФИЗ-, но не ЛПС-группы, после содержания в ОС по сравнению со СТАНД-условиями большую экспрессию мРНК противовоспалительного цитокина TGF-beta1 в дорзальном гиппокампе, миндалине и провоспалительного цитокина IL-1beta в префронтальной коре. Таким образом, содержание в ОС оказывало благотворное влияние на поведение крыс ЛПС-группы, уменьшая доминантные свойства и агрессивное поведение.

early proinflammatory stresslipopolysaccharidesocial dominance in the tuberesident-intruder testenvironment enrichmentexpression of mRNA genes of proinflammatory and anti-inflammatory cytokinesранний провоспалительный стресслипополисахаридсоциальное доминирование в трубетест резидент-интрудеробогащение средыэкспрессия мРНК генов провоспалительных и противовоспалительных цитокиновПравительство РФGovernment of the Russian Federation1021062411613-6-3.1.4.Брошевицкая Н.Д., Павлова И.В., Зайченко М.И. Ранний провоспалительный стресс влияет на социальное поведение взрослых крыс: эффекты пола и базового уровня интерлейкина 1-бета в крови. Нейрохимия. 2022. 39(3): 279–287.Павлова И.В., Брошевицкая Н.Д. Влияние активации иммунной системы в раннем онтогенезе на агрессивность и сексуальную мотивацию у взрослых крыс Вистар. Рос. физиол. журн. им. И.М. Сеченова. 2023. 109(10): 1476–1488.Abou-Ismail U.A. 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