Intrinsically disordered regions affect the expression of Toll-like receptor TIR domains in soluble form
- 作者: Lushpa V.A.1, Goncharuk М.V.1, Arseniev A.S.1, Mineev К.S.1,2, Goncharuk S.A.1
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隶属关系:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt
- 期: 卷 51, 编号 3 (2025)
- 页面: 523-530
- 栏目: ПИСЬМА РЕДАКТОРУ
- URL: https://innoscience.ru/0132-3423/article/view/687051
- DOI: https://doi.org/10.31857/S0132342325030145
- EDN: https://elibrary.ru/KRUWDK
- ID: 687051
如何引用文章
详细
Here we investigate the effect of the C-terminal region on the solubility of TLR4 and TLR10 TIR domains upon the heterologous expression in Escherichia coli. We demonstrated that the absence of this region significantly enhances the yields of globular domains in a soluble form. These findings complement the protocols, previously established to synthesize the TLR TIR domains for the structural studies by various techniques, including by NMR spectroscopy.
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作者简介
V. Lushpa
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: ms.goncharuk@gmail.com
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 142290
М. Goncharuk
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: ms.goncharuk@gmail.com
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 142290
A. Arseniev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: ms.goncharuk@gmail.com
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 142290
К. Mineev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt
Email: ms.goncharuk@gmail.com
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 142290; Max-von-Laue-Strasse 9, Frankfurt am Main, 60438 Germany
S. Goncharuk
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
编辑信件的主要联系方式.
Email: ms.goncharuk@gmail.com
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 142290
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