Fluke worm Azygia lucii and pike Esox lucius: features of the relationship

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The effect of Azygia lucii fluke infection on the activity of proteolytic enzymes functioning in the stomach of the host pike and the ability of worms to suppress this activity was studied. The activity of a wide range of proteases was detected in the pike’s stomach. In extracts of marita A. lucii, both pepsin-like activity and activity of alkaline proteases, a significant part of which are metal-dependent proteases, were determined. Even with a low intensity of invasion, trematodes cause an increase in the activity of pepsin-like proteases in the host’s stomach. Worm extract suppresses the activity of commercial pepsin, but neither the incubation medium nor the trematode extract have a statistically significant inhibitory effect on the activity of pepsin-like proteases of the gastric mucosa of pike.

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Sobre autores

T. Frolova

Papanin Institute for Biology of Inland Waters

Autor responsável pela correspondência
Email: bianka28061981@gmail.com
Rússia, Borok, Yaroslavl Region, 152742

A. Sokolova

Papanin Institute for Biology of Inland Waters

Email: bianka28061981@gmail.com
Rússia, Borok, Yaroslavl Region, 152742

G. Izvekova

Papanin Institute for Biology of Inland Waters

Email: bianka28061981@gmail.com
Rússia, Borok, Yaroslavl Region, 152742

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2. Fig. 1. Inhibitory analysis of proteases from Azygia lucii marit, n = 5.

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3. Fig. 2. Pepsin-like activity of the gastric mucosa of uninfected (I), infected (II) pikes and the extract of the trematode Azygia lucii (III), n = 5. * indicates the reliability of the difference between infected pikes relative to uninfected ones.

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4. Fig. 3. Effect of pH of the medium on the total proteolytic activity of the gastric mucosa of pike, n = 5.

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5. Fig. 4. Inhibitory analysis of proteases functioning in the gastric mucosa of pike, n = 5.

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6. Fig. 5. The effect of the incubation medium and the extract of Azygia lucii on the activity of pepsin (a) and the pepsin-like activity of the gastric mucosa of pike (b), n = 5. * indicates the reliability of the difference of the extract relative to the control.

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