Study of the gut transcriptomic response in Drosophila melanogaster with knockdown of the Gagr, domesticated gag/i> gene of errantiviruses

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Аннотация

As a result of molecular domestication of the gag gene of errantiviruses, the Gagr gene was formed in the genome of Drosophila melanogaster. It has previously been shown that the Gagr gene is transcribed at the highest level in gut tissues relative to other tissues, and its transcription is most effectively induced in females in response to ammonium persulfate added to the diet. In the present work, the gut transcriptome of females with knockdown of the Gagr gene was studied in all tissues under standard conditions and under stress conditions caused by ammonium persulfate. It was revealed that in females with knockdown of the Gagr gene, the genes of animicrobial peptides controlled by the Toll and Imd signaling pathways are activated in the gut. Induction of a stress response by ammonium persulfate revealed disruption of the JAK/STAT and JNK/MAPK signaling pathways and an almost complete absence of activation of the ER-stress and UPR-stress pathways in the Gagr gene mutant. The data obtained confirm the important role of the Gagr gene in maintaining the homeostasis and the immune response.

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Авторлар туралы

M. Nikitina

Faculty of Biology, Lomonosov Moscow State University

Email: nefedova@mail.bio.msu.ru
Ресей, Moscow, 119234

P. Milyaeva

Faculty of Biology, Lomonosov Moscow State University

Email: nefedova@mail.bio.msu.ru
Ресей, Moscow, 119234

I. Kuzmin

Faculty of Biology, Lomonosov Moscow State University

Email: nefedova@mail.bio.msu.ru
Ресей, Moscow, 119234

L. Nefedova

Faculty of Biology, Lomonosov Moscow State University

Хат алмасуға жауапты Автор.
Email: nefedova@mail.bio.msu.ru
Ресей, Moscow, 119234

Әдебиет тізімі

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9. Fig. 1. Comparative analysis of intestinal transcriptomes of Gagr knockdown and control females. a – Heat map and cluster analysis of intestinal transcriptomes of Gagr knockdown and control females cultured under standard conditions and on APS-containing medium. b – Enrichment of functional categories of genes with differential expression (|LFC| > 2) in the intestine of Gagr knockdown females relative to the control line. The abscissa scale shows the negative logarithm of the P-value for a given category. c – Enrichment of functional categories of genes with increased and decreased transcription during stress response (|LFC| > 2) in control and Gagr knockdown females. (g) Venn diagrams showing the number of common genes whose transcription was altered by stress response in control (C) and Gagr knockdown (Gagr-RNAi) females and their enrichment in functional categories.

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10. Fig. 2. Enrichment of functional categories of genes with increased transcription (LFC > 2) in the intestine of control females and unchanged or decreased transcription in the intestine of Gagr knockdown females (LFC < 0.5). The cluster network of functional categories of genes constructed in the Metascape program is shown below the diagram (explanations in the text).

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11. Fig. 3. Transcriptional response of the genes of the JNK/MAPK, JAK/STAT, and ER stress signaling pathways in response to ammonium persulfate in the intestines of control females (a) and females with Gagr knockdown (b). To construct the signaling pathway diagrams, data from [20] and [23] were used. Genes whose transcription increased more than 2-fold are shown in red. Genes whose expression increased 1.5–2-fold are highlighted in pink. The LFC value is given in brackets near the genes, kinases are highlighted in bold; xbp1s is the spliced ​​form of xbp1. *TotA – expression of this gene in individuals with knockdown is lower than in the control, but is induced during the stress response; **hid – expression of this gene in individuals with Gagr knockdown is higher than in the control, and is not induced during the stress response.

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